01:146:475 Immunology Laboratory
This course may be used to fulfill the elective and laboratory requirements of the Biological Sciences major.
Prerequisite: 01:119:117 or 01:119:102 Pre- or Corequisite: Immunology 01:146:474
The overall objective of this course is to give the advanced biology student experience with addressing immunological questions through experimentation. A representative experiment will be the analysis of different lymphocyte subsets in distinct lymphoid tissues of the mouse. Specific techniques will analyze RNA and protein expression in different immune cell populations and explore the functions of these unique cells.
Representative Syllabus (may be slightly modified year to year):
Title of Lab
1. Isolation of mouse spleen mononuclear cells by density gradient centrifugation
2. Natural resistance to infection and isolation of lymphoid tissues
3. Cytotoxic activity of an activated macrophage cell line
4. Characterization of T cell populations isolated from mouse spleen and thymus
5. Flow cytometry analysis of cells
6. Preparation of RNA from mouse tissues
7. RT-PCR as a method for identifying CD4 expression in different murine lymphoid tissues
8. Western blot analysis of a B cell-specific protein in human B cells
Sakai website will be active during the semester
Course Satisfies Learning Goals
1. Master factual and conceptual knowledge in Immunology that will provide a solid foundation for success in advanced training and professional careers.
2. Develop an ability to summarize, integrate and organize information and relate it to disease outcomes
3. Use scientific reasoning to evaluate the potential for current research and new discoveries to improve our understanding of immunology and its relevance to human health and to our society.
4. Use experimental techniques to address immunological questions.
Exams, Assignments, and Grading Policy
Grades are weighted most heavily on four lab reports and/or presentation. Attendance and participation in the labs are also factors in the final grade. There will also be a quiz that tests the principles of Lab Assignment #1.
If this course is closed, please use the following link to add your name to the wait list: Wait List Sign Up for Spring 2017 Courses If you have any questions, please contact the Division of Life Sciences - Office of Undergraduate Instruction at 848-445-2075 or visit our office at Nelson Biological Laboratories B112, Busch Campus.
B314 Nelson Hall
Other Faculty Participants:
B336 Nelson Labs
B319 Nelson Labs
D411 Nelson Hall
B303 Nelson Hall
** All information is subject to change at the discretion of the course coordinator.